plasma c reactive protein crp levels  (R&D Systems)

Journal: bioRxiv

Article Title: GP130 Y814 SIGNALING IS REQUIRED FOR THE DYNAMIN-MEDIATED ENDOCYTOSIS, MAPK/P38 ACTIVATION AND PERSISTENCE OF CHRONIC SYSTEMIC INFLAMMATION INDUCED BY HIGH FAT DIET

doi: 10.64898/2026.02.06.704505

Figure Lengend Snippet: (A) Schematic of experimental design. (B) Weight change over time of mice on high fat diet (HFD), n=10 biological replicates per group. (C) Representative images and body composition analysis of WT mice on normal diet (ND) or HFD, generated by DEXA scan. (D) Serum levels of IL-6, CRP, and MCP-1 measured by ELISA in mice fed ND and HFD for 42 weeks. (E) Circulating lymphocytes, neutrophils, and monocytes in peripheral blood from mice on HFD as detected by white blood cell differential. Data points represent biological replicates, error bars are shown as mean +/- SEM, and statistical analyses were performed with 2-way ANOVA with Fisher’s LSD test for more than two groups or Student’s t-test for comparing two groups; p-values less than 0.05 were considered significant.

Article Snippet: ELISA kits (mouse CRP (MCRP00), mouse IL-6 (M6000B), mouse CCL2/MCP-1 (MJE00B)) were purchased from R&D Systems and performed following the manufacturer’s instruction.

Techniques: Generated, Enzyme-linked Immunosorbent Assay

Journal: Microorganisms

Article Title: Effects of Porphyromonas gingivalis Bacteria on Inflammation, Oxidative Stress and Lipid Metabolism in Models of Obese db / db Mice and 3T3-L1 Adipose Cells

doi: 10.3390/microorganisms13092074

Figure Lengend Snippet: Effect of P. gingivalis bacteria on plasma and adipose tissue inflammatory markers in obese db/db mice. Obese db/db mice were intravenously injected with vehicle (Control) or P. gingivalis bacteria ( P. g bacteria) at 10 7 CFU. After 4 h, CRP levels in plasma ( a ) and liver ( b ) were measured by ELISA kit. Levels of IL-6, MCP-1 and TNFα were determined in subcutaneous (SAT, ( c – e )) and visceral (VAT, ( f – h )) adipose tissues by ELISA kits. Data are expressed as mean ± SEM ( n = 5). * p < 0.05, ** p < 0.01, *** p < 0.005 as compared to Control.

Article Snippet: Cholesterol and CRP levels were evaluated in both plasma and liver by using Total Cholesterol Colorimetric/Fluorometric assay kit (26-K957, Biovision) and specific Mouse CRP ELISA kit (MCRP00, R&D systems, Minneapolis, MN, USA), respectively.

Techniques: Bacteria, Clinical Proteomics, Injection, Control, Enzyme-linked Immunosorbent Assay

Journal: Microorganisms

Article Title: Effects of Porphyromonas gingivalis Bacteria on Inflammation, Oxidative Stress and Lipid Metabolism in Models of Obese db / db Mice and 3T3-L1 Adipose Cells

doi: 10.3390/microorganisms13092074

Figure Lengend Snippet: Effect of P. gingivalis bacteria and LPS on the secretion of adipokines from 3T3-L1 adipocytes. Differentiated adipocytes were exposed or not to P. gingivalis bacteria or LPS for 48 h, and adipocytes were differentiated in the presence or not of P. gingivalis bacteria or LPS for 12 d. The levels of IL-6 ( a – f ), MCP-1 ( b – g ), leptin ( c – h ), resistin ( d – i ) and adiponectin ( e – j ) secreted by adipocytes were measured by specific ELISA kits. Data were expressed as mean ± SEM of n = 4 independent experiments for the acute condition and n = 3 independent experiments for the chronic condition. * p < 0.05, ** p < 0.01, *** p < 0.005 as compared to Control.

Article Snippet: Cholesterol and CRP levels were evaluated in both plasma and liver by using Total Cholesterol Colorimetric/Fluorometric assay kit (26-K957, Biovision) and specific Mouse CRP ELISA kit (MCRP00, R&D systems, Minneapolis, MN, USA), respectively.

Techniques: Bacteria, Enzyme-linked Immunosorbent Assay, Control